User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03

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September 3, 2013

Objective

Execute Dr Hartings' procedureby making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW:267.24g/mol<br.> Inosine MW:268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL) 0.0082M<br.> Inosine: (from 0.1060g in 50mL) 0.0079M<br.> <br.> Dilutions to make:<br.>

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5
RANDOM: 0.25x10-5 RANDOM: 0.40x10-5

Data

Absorption Spectra

Calculations and Analysis

Determine the standard deviation for your data points.<br.> Determine the confidence interval for 90% and 95% confidence.<br.> Determine if any data can be ruled out using a Q-test.<br.>

Calibration Curves

Determining Unknown's Concentration

Notes


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