User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03

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(Determining Unknown's Concentration)
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[[Image:2013_0903_adenosine_calibration.PNG]]
[[Image:2013_0903_adenosine_calibration.PNG]]
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=====Determining Unknown's Concentration=====
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*Unknown from:
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*Concentration determined from calibration curve:
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*Actual concentration:
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===Measurement Notes===
===Measurement Notes===

Revision as of 13:21, 4 September 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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September 3, 2013

Objective

Execute procedure for making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW: 267.24g/mol
Inosine MW: 268.2g/mol

Original stock solutions:
Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M
Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M

Dilutions to make, each with total volumes of 1mL:

Adenosine solution concentrations (M) Volume stock solution added (μL) Inosine solution concentrations (M) Volume of stock solution added (μL)
3.00x10-5 3.66 4.80x10-5 6.08
2.50x10-5 3.05 4.00x10-5 5.06
2.00x10-5 2.44 3.20x10-5 4.05
1.50x10-5 1.83 2.40x10-5 3.04
1.00x10-5 1.22 1.60x10-5 2.03
0.50x10-5 0.610 0.80x10-5 0.101
RANDOM: 0.25x10-5 0.305 RANDOM: 0.40x10-5 0.506

Data

Absorption Spectra

Image:2013_0903_adenosine_spectra.PNG
All peaks at 259nm.

Calculations and Analysis

Determine the standard deviation for your data points.
Determine the confidence interval for 90% and 95% confidence.
Determine if any data can be ruled out using a Q-test.

Calibration Curves

Image:2013_0903_adenosine_calibration.PNG


Measurement Notes

  • Microliter amounts were measured with a micropipet
  • 50mL volumetric flasks were used to make stock solutions of both adenosine and inosine



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