User:Madeleine Y. Bee/Notebook/CHEM571 2013F/2013/09/03
From OpenWetWare
(Difference between revisions)
Line 13:  Line 13:  
===Procedure Prep===  ===Procedure Prep===  
  ====Stock Solutions and Dilutions====  +  =====Stock Solutions and Dilutions===== 
Adenosine MW: 267.24g/mol<br.>  Adenosine MW: 267.24g/mol<br.>  
Inosine MW: 268.2g/mol<br.>  Inosine MW: 268.2g/mol<br.>  
Line 65:  Line 65:  
===Data===  ===Data===  
  ====Absorption Spectra====  +  =====Absorption Spectra===== 
[[Image:2013_0903_adenosine_spectra.PNG]]<br.>  [[Image:2013_0903_adenosine_spectra.PNG]]<br.>  
All peaks at 259nm.  All peaks at 259nm.  
Line 73:  Line 73:  
Determine the confidence interval for 90% and 95% confidence.<br.>  Determine the confidence interval for 90% and 95% confidence.<br.>  
Determine if any data can be ruled out using a Qtest.<br.>  Determine if any data can be ruled out using a Qtest.<br.>  
  ====Calibration Curves====  +  =====Calibration Curves===== 
[[Image:2013_0903_adenosine_calibration.PNG]]  [[Image:2013_0903_adenosine_calibration.PNG]]  
  ====Determining Unknown's Concentration====  +  =====Determining Unknown's Concentration===== 
*Unknown from:  *Unknown from:  
*Concentration determined from calibration curve:  *Concentration determined from calibration curve: 
Revision as of 15:38, 3 September 2013
Experimental Biological Chemistry: Fall 2013  Main project page Previous entry Next entry  
September 3, 2013ObjectiveExecute procedure for making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves. Procedure PrepStock Solutions and DilutionsAdenosine MW: 267.24g/mol
DataAbsorption SpectraCalculations and AnalysisDetermine the standard deviation for your data points. Calibration CurvesDetermining Unknown's Concentration
Measurement Notes
