User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objective)
Line 10: Line 10:
===Objective===
===Objective===
Execute Dr Hartings' [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure] by
Execute Dr Hartings' [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure] by
-
making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.  
+
making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.
 +
 
===Procedure Prep===
===Procedure Prep===
====Stock Solutions and Dilutions====
====Stock Solutions and Dilutions====

Revision as of 15:24, 3 September 2013

Experimental Biological Chemistry: Fall 2013 Main project page
Previous entry      Next entry

September 3, 2013

Objective

Execute Dr Hartings' procedure by making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW: 267.24g/mol
Inosine MW: 268.2g/mol

Original stock solutions:
Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M
Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M

Dilutions to make, each with total volumes of 1mL:

Adenosine solution concentrations (M) Volume stock solution added (L) Inosine solution concentrations (M) Volume of stock solution added (L)
3.00x10-5 3.66x10-6 4.80x10-5 6.08x10-6
2.50x10-5 3.05x10-6 4.00x10-5 5.06x10-6
2.00x10-5 2.44x10-6 3.20x10-5 4.05x10-6
1.50x10-5 1.83x10-6 2.40x10-5 3.04x10-6
1.00x10-5 1.22x10-6 1.60x10-5 2.03x10-6
0.50x10-5 6.10x10-7 0.80x10-5 1.01x10-6
RANDOM: 0.25x10-5 3.05x10-7 RANDOM: 0.40x10-5 5.06x10-7

Data

Absorption Spectra

Image:2013_0903_adenosine_spectra.PNG

Calculations and Analysis

Determine the standard deviation for your data points.
Determine the confidence interval for 90% and 95% confidence.
Determine if any data can be ruled out using a Q-test.

Calibration Curves

Image:2013_0903_adenosine_calibration.PNG

Determining Unknown's Concentration


Personal tools