User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03

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(Calibration Curves)
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==September 3, 2013==
==September 3, 2013==
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===Objective===
===Objective===
Execute Dr Hartings' [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure]by
Execute Dr Hartings' [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure]by
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====Determining Unknown's Concentration====
====Determining Unknown's Concentration====
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===Notes===
 

Revision as of 15:12, 3 September 2013

Experimental Biological Chemistry: Fall 2013 Main project page
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September 3, 2013

Objective

Execute Dr Hartings' procedureby making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW:267.24g/mol
Inosine MW:268.2g/mol

Original stock solutions:
Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M
Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M

Dilutions to make, each with total volumes of 1mL:

Adenosine solution concentrations (M) Volume stock solution added (L) Inosine solution concentrations (M) Volume of stock solution added (L)
3.00x10-5 3.66x10-6 4.80x10-5 6.08x10-6
2.50x10-5 3.05x10-6 4.00x10-5 5.06x10-6
2.00x10-5 2.44x10-6 3.20x10-5 4.05x10-6
1.50x10-5 1.83x10-6 2.40x10-5 3.04x10-6
1.00x10-5 1.22x10-6 1.60x10-5 2.03x10-6
0.50x10-5 6.10x10-7 0.80x10-5 1.01x10-6
RANDOM: 0.25x10-5 3.05x10-7 RANDOM: 0.40x10-5 5.06x10-7

Data

Absorption Spectra

Image:2013_0903_adenosine_spectra.PNG

Calculations and Analysis

Determine the standard deviation for your data points.
Determine the confidence interval for 90% and 95% confidence.
Determine if any data can be ruled out using a Q-test.

Calibration Curves

Image:2013_0903_adenosine_calibration.PNG

Determining Unknown's Concentration


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