User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03: Difference between revisions

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==September 3, 2013==
==September 3, 2013==
===Objective===
===Objective===
Execute Dr Hartings' [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure] by
Execute [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03 procedure] for
making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.
making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.


===Procedure Prep===
===Procedure Prep===
====Stock Solutions and Dilutions====
=====Stock Solutions and Dilutions=====
Adenosine MW: 267.24g/mol<br.>
Adenosine MW: 267.24g/mol<br.>
Inosine MW: 268.2g/mol<br.>
Inosine MW: 268.2g/mol<br.>
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{|style="width:700px"
{|style="width:700px"
|<u>Adenosine solution concentrations (M)</u>
|<u>Adenosine solution concentrations (M)</u>
|<u>Volume stock solution added (L)</u>
|<u>Volume stock solution added (μL)</u>
|<u>Inosine solution concentrations (M)</u>
|<u>Inosine solution concentrations (M)</u>
|<u>Volume of stock solution added (L)</u>
|<u>Volume of stock solution added (μL)</u>
|-
|-
|3.00x10<sup>-5</sup>
|3.00x10<sup>-5</sup>
|3.66x10<sup>-6</sup>
|3.66
|4.80x10<sup>-5</sup>
|4.80x10<sup>-5</sup>
|6.08x10<sup>-6</sup>
|6.08
|-  
|-  
|2.50x10<sup>-5</sup>  
|2.50x10<sup>-5</sup>  
|3.05x10<sup>-6</sup>
|3.05
|4.00x10<sup>-5</sup>
|4.00x10<sup>-5</sup>
|5.06x10<sup>-6</sup>
|5.06
|-  
|-  
|2.00x10<sup>-5</sup>  
|2.00x10<sup>-5</sup>  
|2.44x10<sup>-6</sup>
|2.44
|3.20x10<sup>-5</sup>
|3.20x10<sup>-5</sup>
|4.05x10<sup>-6</sup>
|4.05
|-  
|-  
|1.50x10<sup>-5</sup>  
|1.50x10<sup>-5</sup>  
|1.83x10<sup>-6</sup>
|1.83
|2.40x10<sup>-5</sup>
|2.40x10<sup>-5</sup>
|3.04x10<sup>-6</sup>
|3.04
|-  
|-  
|1.00x10<sup>-5</sup>  
|1.00x10<sup>-5</sup>  
|1.22x10<sup>-6</sup>
|1.22
|1.60x10<sup>-5</sup>
|1.60x10<sup>-5</sup>
|2.03x10<sup>-6</sup>
|2.03
|-  
|-  
|0.50x10<sup>-5</sup>  
|0.50x10<sup>-5</sup>  
|6.10x10<sup>-7</sup>
|0.610
|0.80x10<sup>-5</sup>
|0.80x10<sup>-5</sup>
|1.01x10<sup>-6</sup>
|0.101
|-  
|-  
|RANDOM: 0.25x10<sup>-5</sup>  
|RANDOM: 0.25x10<sup>-5</sup>  
|3.05x10<sup>-7</sup>
|0.305
|RANDOM: 0.40x10<sup>-5</sup>  
|RANDOM: 0.40x10<sup>-5</sup>  
|5.06x10<sup>-7</sup>
|0.506
|}
|}


===Data===
===Data===
====Absorption Spectra====
=====Absorption Spectra=====
[[Image:2013_0903_adenosine_spectra.PNG]]<br.>
[[Image:2013_0904_adenosine_spectra_(rerun).PNG]]<br.>
All peaks at 259nm.
All peaks at 259nm.


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Determine the confidence interval for 90% and 95% confidence.<br.>
Determine the confidence interval for 90% and 95% confidence.<br.>
Determine if any data can be ruled out using a Q-test.<br.>
Determine if any data can be ruled out using a Q-test.<br.>
====Calibration Curves====
=====Calibration Curves=====
[[Image:2013_0903_adenosine_calibration.PNG]]
[[Image:2013_0903_adenosine_calibration.PNG]]


====Determining Unknown's Concentration====
===Measurement Notes===
*Unknown from:
*Microliter amounts were measured with a micropipet
*Concentration determined from calibration curve:
*50mL volumetric flasks were used to make stock solutions of both adenosine and inosine
*Actual concentration:
 
 
 
 
 
 




Revision as of 11:44, 4 September 2013

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September 3, 2013

Objective

Execute procedure for making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW: 267.24g/mol<br.> Inosine MW: 268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M<br.> Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M<br.> <br.> Dilutions to make, each with total volumes of 1mL:<br.>

Adenosine solution concentrations (M) Volume stock solution added (μL) Inosine solution concentrations (M) Volume of stock solution added (μL)
3.00x10-5 3.66 4.80x10-5 6.08
2.50x10-5 3.05 4.00x10-5 5.06
2.00x10-5 2.44 3.20x10-5 4.05
1.50x10-5 1.83 2.40x10-5 3.04
1.00x10-5 1.22 1.60x10-5 2.03
0.50x10-5 0.610 0.80x10-5 0.101
RANDOM: 0.25x10-5 0.305 RANDOM: 0.40x10-5 0.506

Data

Absorption Spectra

<br.> All peaks at 259nm.

Calculations and Analysis

Determine the standard deviation for your data points.<br.> Determine the confidence interval for 90% and 95% confidence.<br.> Determine if any data can be ruled out using a Q-test.<br.>

Calibration Curves

Measurement Notes

  • Microliter amounts were measured with a micropipet
  • 50mL volumetric flasks were used to make stock solutions of both adenosine and inosine



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