User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03: Difference between revisions
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*Concentration determined from calibration curve: | *Concentration determined from calibration curve: | ||
*Actual concentration: | *Actual concentration: | ||
===Measurement Notes=== | |||
*Microliter amounts were measured with a micropipet | |||
*50mL volumetric flasks were used to make stock solutions of both adenosine and inosine | |||
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Revision as of 12:37, 3 September 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||
September 3, 2013ObjectiveExecute procedure for making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves. Procedure PrepStock Solutions and DilutionsAdenosine MW: 267.24g/mol<br.> Inosine MW: 268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M<br.> Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M<br.> <br.> Dilutions to make, each with total volumes of 1mL:<br.>
DataAbsorption SpectraCalculations and AnalysisDetermine the standard deviation for your data points.<br.> Determine the confidence interval for 90% and 95% confidence.<br.> Determine if any data can be ruled out using a Q-test.<br.> Calibration CurvesDetermining Unknown's Concentration
Measurement Notes
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