User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03: Difference between revisions

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Inosine MW:268.2g/mol<br.>
Inosine MW:268.2g/mol<br.>
<br.>
<br.>
Stock solutions to make:<br.>
Original stock solutions:<br.>
Adenosine: (from 0.1091g in 50mL) 0.0082M<br.>
Inosine: (from 0.1060g in 50mL) 0.0079M<br.>
<br.>
Dilutions to make:<br.>
{|style="width:700px"
{|style="width:700px"
|<u>Adenosine solution concentrations (M)</u>
|<u>Adenosine solution concentrations (M)</u>

Revision as of 10:15, 3 September 2013

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September 3, 2013

Objective

Execute Dr Hartings' procedureby making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW:267.24g/mol<br.> Inosine MW:268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL) 0.0082M<br.> Inosine: (from 0.1060g in 50mL) 0.0079M<br.> <br.> Dilutions to make:<br.>

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5
RANDOM: 0.25x10-5 RANDOM: 0.40x10-5

Data

Absorption Spectra

Calculations and Analysis

Calibration Curves

Determining Unknown's Concentration

Notes


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