User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> | Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> | ||
===Data=== | ===Data and Calculations=== | ||
[[Image:2013_0828_citrate_AuNPs.png]]<br.> | |||
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> | Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> | ||
*Relevant wavelengths and absorption: | |||
λ<sub>spr</sub>=518.5nm<br.> | λ<sub>spr</sub>=518.5nm<br.> | ||
A<sub>spr</sub>=0.143<br.> | A<sub>spr</sub>=0.143<br.> | ||
A<sub>450</sub>=0.091<br.> | A<sub>450</sub>=0.091<br.> | ||
A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.> | A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.> | ||
According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.> | *According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.> | ||
AuNP diameter=12nm<br.> | AuNP diameter=12nm<br.> | ||
ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.> | ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.> | ||
Using Beer's Law to calculate concentration:<br.> | *Using Beer's Law to calculate concentration:<br.> | ||
C=1.312E-09M or 1.312nM<br.> | C=1.312E-09M or 1.312nM<br.> | ||
Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> | *Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> | ||
C=13.12nM<br.> | C=13.12nM<br.> | ||
===Notes=== | ===Notes=== | ||
Revision as of 13:38, 28 August 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
August 28, 2013ObjectiveExecute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook Procedures and CalculationsBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> Rest of procedure followed as written. Citrate-AuNP Procedure[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> Rest of procedure followed as written.<br.> Final volume AuNPs: 26.5mL<br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> Data and Calculations<br.> Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
λspr=518.5nm<br.> Aspr=0.143<br.> A450=0.091<br.> Aspr/A450=0.143/0.091=1.57<br.>
AuNP diameter=12nm<br.> ε450 for 12nm AuNPs=1.09E+08<br.>
C=1.312E-09M or 1.312nM<br.>
C=13.12nM<br.> Notes |