User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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Rest of procedure followed as written. | Rest of procedure followed as written. | ||
====Citrate-AuNP Procedure==== | ====Citrate-AuNP Procedure==== | ||
[HAuCl<sub>4</sub>]=0.249Mm<BR.> | |||
Sodium citrate=1.01%<br.> | |||
Rest of procedure followed as written.<br.> | |||
Final volume AuNPs: 26.5mL<br.> | |||
Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> | |||
===Data=== | ===Data=== | ||
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> | Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> | ||
<br.> | <br.> | ||
[[Image:2013_0828_citrate_AuNPs.png]] | [[Image:2013_0828_citrate_AuNPs.png]]<br.> | ||
<br.> | |||
λ<sub>spr</sub>=518.5nm<br.> | |||
A<sub>spr</sub>=0.143<br.> | |||
A<sub>450</sub>=0.091<br.> | |||
A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.> | |||
According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.> | |||
AuNP diameter=12nm<br.> | |||
ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.> | |||
Using Beer's Law to calculate concentration:<br.> | |||
C=1.312E-09M or 1.312nM<br.> | |||
Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> | |||
C=13.12nM<br.> | |||
===Notes=== | ===Notes=== | ||
Revision as of 13:34, 28 August 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
August 28, 2013ObjectiveExecute procedure from from Dr Harting's AU Biomaterials Design Lab Notebook DescriptionBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> Rest of procedure followed as written. Citrate-AuNP Procedure[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> Rest of procedure followed as written.<br.> Final volume AuNPs: 26.5mL<br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.> DataAbsorbance spectra for AuNPs synthesized by citrate reduction.<br.> <br.> <br.> <br.> λspr=518.5nm<br.> Aspr=0.143<br.> A450=0.091<br.> Aspr/A450=0.143/0.091=1.57<br.> According to the Supporting Information from 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra':<br.> AuNP diameter=12nm<br.> ε450 for 12nm AuNPs=1.09E+08<br.> Using Beer's Law to calculate concentration:<br.> C=1.312E-09M or 1.312nM<br.> Accounting for the 10x dilution factor used in taking absorbance measurements:<br.> C=13.12nM<br.> Notes |