User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions

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[BSA]=15.6μM<br.>
[BSA]=15.6μM<br.>
final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.>
final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.>
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)V<br.>
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)(V)<br.>
V=1.809mL BSA<br.>
V=1.809mL BSA<br.>
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.>
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.>

Revision as of 12:18, 28 August 2013

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August 28, 2013

Objective

Execute procedure from from Dr Harting's AU Biomaterials Design Lab Notebook

Description

BSA-AuNP Procedure

[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> Rest of procedure followed as written.

Citrate-AuNP Procedure

Data

Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> <br.>

Notes


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