User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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Execute procedure from from [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr Harting's AU Biomaterials Design Lab Notebook] | Execute procedure from from [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr Harting's AU Biomaterials Design Lab Notebook] | ||
===Description=== | ===Description=== | ||
====BSA-AuNP Procedure==== | |||
[HAuCl<sub>4</sub>]=2.54nM<br.> | |||
[BSA]=15.6μM<br.> | |||
final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.> | |||
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)V<br.> | |||
V=1.809mL BSA<br.> | |||
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.> | |||
Rest of procedure followed as written. | |||
====Citrate-AuNP Procedure==== | |||
===Data=== | ===Data=== | ||
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.> | |||
<br.> | |||
[[Image:2013_0828_citrate_AuNPs.png]] | |||
===Notes=== | ===Notes=== | ||
Revision as of 12:16, 28 August 2013
 Experimental Biological Chemistry: Fall 2013
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August 28, 2013ObjectiveExecute procedure from from Dr Harting's AU Biomaterials Design Lab Notebook DescriptionBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)V<br.> V=1.809mL BSA<br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> Rest of procedure followed as written. Citrate-AuNP ProcedureDataAbsorbance spectra for AuNPs synthesized by citrate reduction.<br.>
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