User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions

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Execute procedures from from [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr Harting's AU Biomaterials Design Lab Notebook]
Execute procedures from from [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr Harting's AU Biomaterials Design Lab Notebook]


===Procedures and Calculations===
===Procedures===
====BSA-AuNP Procedure====
====BSA-AuNP Procedure====
[HAuCl<sub>4</sub>]=2.54nM<br.>
[HAuCl<sub>4</sub>]=2.54nM<br.>
[BSA]=15.6μM<br.>
[BSA]=15.6μM<br.>
final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.>
Final [HAuCl<sub>4</sub>]: 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.>
<br.>
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)(V)<br.>
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)(V)<br.>
V=1.809mL BSA<br.>
V=1.809mL BSA<br.>
<br.>
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.>
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.>
<br.>
Rest of procedure followed as written.
Rest of procedure followed as written.
====Citrate-AuNP Procedure====
====Citrate-AuNP Procedure====
[HAuCl<sub>4</sub>]=0.249Mm<BR.>
[HAuCl<sub>4</sub>]=0.249Mm<BR.>
Sodium citrate=1.01%<br.>
Sodium citrate=1.01%<br.>
<br.>
Rest of procedure followed as written.<br.>
Rest of procedure followed as written.<br.>
Final volume AuNPs: 26.5mL<br.>
<br.>
Final volume AuNPs: 25.6mL<br.>
<br.>
Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>
Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>


===Data===
===Data===
[[Image:2013_0828_citrate_AuNPs.png]]<br.>
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>
<br.>
*Relevant wavelengths and absorption:
[[Image:2013_0828_citrate_AuNPs.png]]<br.>
**λ<sub>spr</sub>=518.5nm<br.>
<br.>
**A<sub>spr</sub>=0.143<br.>
λ<sub>spr</sub>=518.5nm<br.>
**A<sub>450</sub>=0.091<br.>
A<sub>spr</sub>=0.143<br.>
**A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.>
A<sub>450</sub>=0.091<br.>
*According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.>
A<sub>spr</sub>/A<sub>450</sub>=0.143/0.091=1.57<br.>
**AuNP diameter=12nm<br.>
According to the Supporting Information from [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra']:<br.>
**ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.>
AuNP diameter=12nm<br.>
*Using Beer's Law to calculate concentration:<br.>
ε<sub>450</sub> for 12nm AuNPs=1.09E+08<br.>
**C=8.349E-10M or 0.8349nM<br.>
Using Beer's Law to calculate concentration:<br.>
*Accounting for the 10x dilution factor used in taking absorbance measurements:<br.>
C=1.312E-09M or 1.312nM<br.>
**C=8.349nM<br.>
Accounting for the 10x dilution factor used in taking absorbance measurements:<br.>
 
C=13.12nM<br.>
===Notes===




Revision as of 10:26, 16 October 2013

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August 28, 2013

Objective

Execute procedures from from Dr Harting's AU Biomaterials Design Lab Notebook

Procedures

BSA-AuNP Procedure

[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> Final [HAuCl4]: 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> <br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> <br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> <br.> Rest of procedure followed as written.

Citrate-AuNP Procedure

[HAuCl4]=0.249Mm<BR.> Sodium citrate=1.01%<br.> <br.> Rest of procedure followed as written.<br.> <br.> Final volume AuNPs: 25.6mL<br.> <br.> Absorbance measurements taken of 10x diluted solution AuNPs rather than original sample concentration.<br.>

Data

<br.> Absorbance spectra for AuNPs synthesized by citrate reduction.<br.>

  • Relevant wavelengths and absorption:
    • λspr=518.5nm<br.>
    • Aspr=0.143<br.>
    • A450=0.091<br.>
    • Aspr/A450=0.143/0.091=1.57<br.>
  • According to the Supporting Information from 'Determination of Size and Concentration of Gold Nanoparticles from UV/vis Spectra':<br.>
    • AuNP diameter=12nm<br.>
    • ε450 for 12nm AuNPs=1.09E+08<br.>
  • Using Beer's Law to calculate concentration:<br.>
    • C=8.349E-10M or 0.8349nM<br.>
  • Accounting for the 10x dilution factor used in taking absorbance measurements:<br.>
    • C=8.349nM<br.>






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