User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/08/28: Difference between revisions
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[BSA]=15.6μM<br.> | [BSA]=15.6μM<br.> | ||
final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.> | final [HAuCl<sub>4</sub>] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl<sub>4</sub>]<br.> | ||
(2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)V<br.> | (2.54mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.404mM HAuCl<sub>4</sub> final)(V)<br.> | ||
V=1.809mL BSA<br.> | V=1.809mL BSA<br.> | ||
Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.> | Combine 1mL HAuCl<sub>4</sub> with 1.810mL BSA.<br.> |
Revision as of 12:18, 28 August 2013
Experimental Biological Chemistry: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
August 28, 2013ObjectiveExecute procedure from from Dr Harting's AU Biomaterials Design Lab Notebook DescriptionBSA-AuNP Procedure[HAuCl4]=2.54nM<br.> [BSA]=15.6μM<br.> final [HAuCl4] 90x [BSA]→15.6μM×90=1404μM=1.404mM=final [HAuCl4]<br.> (2.54mM HAuCl4 initial)(1mL solution)=(1.404mM HAuCl4 final)(V)<br.> V=1.809mL BSA<br.> Combine 1mL HAuCl4 with 1.810mL BSA.<br.> Rest of procedure followed as written. Citrate-AuNP ProcedureDataAbsorbance spectra for AuNPs synthesized by citrate reduction.<br.> <br.> Notes |