User:Mackenzie L. Cowell/IGEM 2007 part fab protocol

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Revision as of 09:08, 9 April 2007 by Mackenzie L. Cowell (talk | contribs) (New page: ==Overview== ==Supply List== The 07 kits will be comprised of no more than 4 384-well plates (1536 parts), so to make 150 kits, we'll need: * '''32 96-deep-well square-bottom plates''' *...)
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Overview

Supply List

The 07 kits will be comprised of no more than 4 384-well plates (1536 parts), so to make 150 kits, we'll need:

  • 32 96-deep-well square-bottom plates
    • 16 for Ed's miniprep, will be sources for the intermediate SPs
    • 16 for miniprepping for Robotic Assembly supplies
  • 16 96-deep-well round-bottom plates (for 2 glycerol stocks of each new registry freezer plate)
  • 56 384-deep-well plates
    • 16 for Antibiotic Testing
    • 4 for Robotic Assembly supplies
    • 36 for intermediate Source Plates for kit fab with the PMP
  • 600 384-well plates, for kits (4 per kit)
  • 672 foil covers
  • 656 plastic covers (AB test plates don't need plastic covers)
  • 15 rolls label tape
  • 32 poxes of tips