User:M Jaffe/Notebook/Maxis Protease Lab 471/2015/09/02

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Objective We created AuNP fibers. More detailed instructions can be found here XXX Procedure Target Concentrations Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions. Stock solutions HAuCl4·H2O 2.5 mM MW = 339.79 g/mol Volume = 50 mL 42.4 mg Lysozyme 20 μM MW = 14307 g/mol Volume = 100 mL 28.6 mg Actual Concentrations The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water. Stock solutions HAuCl4·H2O 50.32 mg MW = 339.79 g/mol Volume = 50 mL 2.96 mM Lysozyme 30.65 mg MW = 14307 g/mol Volume = 100 mL 21.4 μM Directions for making samples Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers. We prepared 16 # 1 mL samples Volume of gold stock: 84.4 μL Volume of protein stock: 259 μL Volume of water: 656 μL We prepared 10 # 5 mL samples Volume of gold stock: 422 μL Volume of protein stock: 1,297 μL Volume of water: 3,281 μL We placed the 16, 1 mL samples and the 10, 5 mL into the oven at 80 degrees Celsius for 4 hours. Data Something must have gone wrong because none of our samples created fibers. We only made nanoparticles. Most likely, the ratio between the protein and the gold was off, which resulted in AuNPs instead of AuNP Fibers.