User:Lymperopoulos Loukas/Notebook/471 LAB/2015/09/16: Difference between revisions
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==Objective== | ==Objective== | ||
1. Centrifuge AuNP fibers | |||
2. Make 500 mL of 10 mM Tris buffer, pH 9 | |||
3. Make 500 mL of 10 mM phosphate buffer, pH 7 | |||
==Description== | ==Description== | ||
Tris buffer | |||
1. calculate the amount needed for 10 mM Tris in 500 ml of water | |||
2. adjust ph with 1 M HCl until ph7 | |||
Phosphate buffer | |||
1. Calculate how much sodium phosphate (dibasic) and sodium phosphate (monobasic) is needed for 10 mM in 500 mL water | |||
==Data== | ==Data== | ||
Revision as of 00:04, 28 November 2015
 Biomaterials Design Lab
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Objective1. Centrifuge AuNP fibers 2. Make 500 mL of 10 mM Tris buffer, pH 9 3. Make 500 mL of 10 mM phosphate buffer, pH 7 DescriptionTris buffer 1. calculate the amount needed for 10 mM Tris in 500 ml of water 2. adjust ph with 1 M HCl until ph7 Phosphate buffer 1. Calculate how much sodium phosphate (dibasic) and sodium phosphate (monobasic) is needed for 10 mM in 500 mL water Data
NotesThis area is for any observations or conclusions that you would like to note.
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