User:Linh N Le/Notebook/2009/08/27

To Do

• We are running low on tips and tubes, so I need to autoclave some today and tomorrow before I leave

Autoclaving

• Doing the rest of the yellow tips in the red boxes
  • about 8 in total
• Also showing Brian how to use the Autoclave

Things that I dont want to fall into obscurity

• Koch mentioned that I should make sure any work I have done does not get forgotten
• I'm pretty sure I have documented all the work I've done
• Here's a list of things that are useful to the lab that I may not want to die:
  1. Inventory Stuff
  2. Profiling laser beams? (it is a bit of an archaic method, but it works on a budget)
• The laser stuff is all on the private wiki, might be a good idea to have it transferred to the public

Inventory'n

• All the inventory stuff is located at http://www.openwetware.org/wiki/Koch_Lab:Inventory
• I even wrote a little blurb at the bottom of this page on how to inventory (which I just updated with more specific details)

Tubulin

• Andy, Brigette and I resuspended some Rhodamine Tubulin today (made 4 aliquots) 1ul in ea aliquot (5mg/ml)
• We then took 1 aliquot of RT and then added it to an aliquot of unlabeled tubulin
  • We have 6, 1ul aliquots that are 15% RT
• I went ahead and polymerized one of the mixed aliquots and we created motility soln
• Andy tried a motility assay, but it failed
  • Brigette and I forgot to add Dextrose to the motility soln and the MT's became "kinked"
• Retrying with dextrose now added
• Note: The 15% RT is enough to see, which is good
• Success! We got to see motility and the MT's are really long and easy to see
• They are moving quite fast and the AF is working great
• No camera (in tweezers lab) so no photo/video footage. Hopefully it works again tomorrow and Andy can bring over the cam