User:Linh N Le/Notebook/2009/08/27

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(Tubulin)
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*Retrying with dextrose now added
*Retrying with dextrose now added
*''Note: The 15% RT is enough to see, which is good
*''Note: The 15% RT is enough to see, which is good
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*Success! We got to see motility and the MT's are really long and easy to see
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Revision as of 18:50, 27 August 2009

Project name Main project page
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To Do

  • We are running low on tips and tubes, so I need to autoclave some today and tomorrow before I leave

Autoclaving

  • Doing the rest of the yellow tips in the red boxes
    • about 8 in total
  • Also showing Brian how to use the Autoclave

Things that I dont want to fall into obscurity

  • Koch mentioned that I should make sure any work I have done does not get forgotten
  • I'm pretty sure I have documented all the work I've done
  • Here's a list of things that are useful to the lab that I may not want to die:
    1. Inventory Stuff
    2. Profiling laser beams? (it is a bit of an archaic method, but it works on a budget)
  • The laser stuff is all on the private wiki, might be a good idea to have it transferred to the public

Inventory'n

Tubulin

  • Andy, Brigette and I resuspended some Rhodamine Tubulin today (made 4 aliquots) 1ul in ea aliquot (5mg/ml)
  • We then took 1 aliquot of RT and then added it to an aliquot of unlabeled tubulin
    • We have 6, 1ul aliquots that are 15% RT
  • I went ahead and polymerized one of the mixed aliquots and we created motility soln
  • Andy tried a motility assay, but it failed
    • Brigette and I forgot to add Dextrose to the motility soln and the MT's became "kinked"
  • Retrying with dextrose now added
  • Note: The 15% RT is enough to see, which is good
  • Success! We got to see motility and the MT's are really long and easy to see
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