User:Linh N Le/Notebook/2009/08/27: Difference between revisions
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*#Profiling laser beams? (it is a bit of an archaic method, but it works on a budget) | *#Profiling laser beams? (it is a bit of an archaic method, but it works on a budget) | ||
*The laser stuff is all on the [http://kochlab.private.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009-1-23/How_to_Profile_a_Laser_with_the_QPD private wiki], might be a good idea to have it transferred to the public | *The laser stuff is all on the [http://kochlab.private.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009-1-23/How_to_Profile_a_Laser_with_the_QPD private wiki], might be a good idea to have it transferred to the public | ||
*My [http://kochlab.private.openwetware.org/wiki/Kochlab_et_al Comic] (on the private wiki). Anyone is allowed to put new comics in, they dont even have to follow the "stick figure" approach is use. | |||
** I have a few ideas that I may need to draw in retroactively, i've just been too busy/lazy to do them lately | |||
===Inventory'n=== | ===Inventory'n=== | ||
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*I even wrote a little blurb at the bottom of this page on how to inventory (which I just updated with more specific details) | *I even wrote a little blurb at the bottom of this page on how to inventory (which I just updated with more specific details) | ||
==Tubulin== | |||
*Andy, Brigette and I resuspended some Rhodamine Tubulin today (made 4 aliquots) 1ul in ea aliquot (5mg/ml) | |||
*We then took 1 aliquot of RT and then added it to an aliquot of unlabeled tubulin | |||
**We have 6, 1ul aliquots that are 15% RT | |||
*I went ahead and polymerized one of the mixed aliquots and we created motility soln | |||
*Andy tried a motility assay, but it failed | |||
**Brigette and I forgot to add Dextrose to the motility soln and the MT's became "kinked" | |||
*Retrying with dextrose now added | |||
*''Note: The 15% RT is enough to see, which is good | |||
*Success! We got to see motility and the MT's are really long and easy to see | |||
*They are moving quite fast and the AF is working great | |||
*No camera (in tweezers lab) so no photo/video footage. Hopefully it works again tomorrow and Andy can bring over the cam | |||
* [[User:Andy Maloney|Andy Maloney]] 23:22, 27 August 2009 (EDT): I'm glad to hear it worked. Did you guys change out the ATP to get it to work or did you use the same stuff? | |||
* [[User:Steven J. Koch|Steve Koch]] 23:48, 27 August 2009 (EDT): Awesome! Glad to hear about motility. I think we'll have a camera soon? | |||
* [[User:Linh N Le|Linh N Le]] 02:30, 28 August 2009 (EDT) We added the dextrose and that fixed all the problems (brigette did most of the work, her notebook will state if she did anything else differently) | |||
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Revision as of 23:37, 27 August 2009
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To Do
Autoclaving
Things that I dont want to fall into obscurity
Inventory'n
Tubulin
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