User:Linh N Le/Notebook/2009/08/26: Difference between revisions

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*[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well
*[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well
**We made both Rhodamine and unlabeled tubulin
**We made both Rhodamine and unlabeled tubulin
*Koch also have [http://openwetware.org/index.php?title=Koch_Lab:Protocols/Kinesin/Tubulin_resuspension_and_polymerization&oldid=307098#Non-labeled_tubulin_resuspension a great page] about resuspending tubulin and polymerizing it
==Procedure==
*The first thing we need is[[Koch Lab:Protocols/GPEM|GPEM]]
*Last time I made 300 microliters total (294 ul [[Koch Lab:Protocols/PEM|PEM]] + 6 ul [[Koch Lab:Protocols/GTP|GTP]])


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Revision as of 13:19, 26 August 2009

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To Do

  • Re-suspension of tubulin rehash

Tubulin

  • Andy needs to know how Brigette, Koch, and I resuspended the tubulin from stock to make the little aliquots that we then polymerize
  • I will try to find all the notes from the wiki from the 3 notebooks of the 3 members above
  • Note: there are instructions that come with the concentrated tubulin
  • My notes say that we resuspended the tubulin on 6/15/2009
  • Koch's notes here as well
    • We made both Rhodamine and unlabeled tubulin
  • Koch also have a great page about resuspending tubulin and polymerizing it

Procedure

  • The first thing we need isGPEM
  • Last time I made 300 microliters total (294 ul PEM + 6 ul GTP)


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