User:Linh N Le/Notebook/2009/08/26: Difference between revisions
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*Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | *Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | ||
**Note: if you want to polymerize one for use that day, no need to flash freeze that tube | **Note: if you want to polymerize one for use that day, no need to flash freeze that tube | ||
*'''Final Concentration 5mg/mL''' | |||
===Unlabeled Tubulin=== | ===Unlabeled Tubulin=== | ||
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*For this, we used the red icebucket and 5 racks to fit all the tubes into the LN2 | *For this, we used the red icebucket and 5 racks to fit all the tubes into the LN2 | ||
*The tubes were then placed into a 50ml falcon tube for storage in the -80C | *The tubes were then placed into a 50ml falcon tube for storage in the -80C | ||
*'''Final Concentration 5mg/ml | |||
==GPEM== | ==GPEM== |
Revision as of 14:26, 27 August 2009
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To Do
Tubulin
ProcedureNote: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start
Rhodamine Tubulin
Unlabeled Tubulin
GPEM
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