User:Linh N Le/Notebook/2009/08/26: Difference between revisions
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*Note: there are instructions that come with the concentrated tubulin | *Note: there are instructions that come with the concentrated tubulin | ||
** [[User:Andy Maloney|Andy Maloney]] 00:58, 27 August 2009 (EDT): Do you know where these are? | ** [[User:Andy Maloney|Andy Maloney]] 00:58, 27 August 2009 (EDT): Do you know where these are? | ||
** [[User:Steven J. Koch|Steve Koch]] 01:07, 27 August 2009 (EDT): There was talk of getting a spikey-thingy and I think Linh even used his own money to get one. If not there, could be in the plastic folder thingy Ant put on the -20C freezer. Or in the cytoskeleton folder? | |||
** [[User:Linh N Le|Linh N Le]] 01:58, 27 August 2009 (EDT) for one, Brigette bought the spike. Secondly, there are old instructions in plastic bags hanging on the side of the small -20 freezer. Third, each "bag of aliquots" that needs resuspending comes with one. Brigette and I read the instructions located on the fridge to make some of these notes | |||
*My notes say that we resuspended the tubulin on [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/15 6/15/2009] | *My notes say that we resuspended the tubulin on [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/15 6/15/2009] | ||
*[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well | *[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well | ||
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*Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | *Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | ||
**Note: if you want to polymerize one for use that day, no need to flash freeze that tube | **Note: if you want to polymerize one for use that day, no need to flash freeze that tube | ||
*'''Final Concentration 5mg/mL''' | |||
===Unlabeled Tubulin=== | ===Unlabeled Tubulin=== | ||
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*For this, we used the red icebucket and 5 racks to fit all the tubes into the LN2 | *For this, we used the red icebucket and 5 racks to fit all the tubes into the LN2 | ||
*The tubes were then placed into a 50ml falcon tube for storage in the -80C | *The tubes were then placed into a 50ml falcon tube for storage in the -80C | ||
*'''Final Concentration 5mg/ml | |||
==GPEM== | ==GPEM== |
Revision as of 14:26, 27 August 2009
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To Do
Tubulin
ProcedureNote: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start
Rhodamine Tubulin
Unlabeled Tubulin
GPEM
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