User:Linh N Le/Notebook/2009/08/26: Difference between revisions
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*The tubes were then placed into a 50ml falcon tube for storage in the -80C | *The tubes were then placed into a 50ml falcon tube for storage in the -80C | ||
==GPEM== | |||
*Brigette and I are going to make a stock of GPEM for use since Andy expressed a want to resuspend tubulin soon | |||
*Following instructions left by [http://www.openwetware.org/wiki/User:Anthony_Salvagno/Notebook/Research/2009/05/12/Microtubule_creation Ant] | |||
*We have PEM premade from before so we will make it with that | |||
*Looking for GTP, notes say that there is 50ul of it in the -20c Freezer | |||
*Koch notes also state that said GTP is make with water and we want to make it with mgcl2 | |||
**There is a tube of premixed MgCl2 on the wet lab shelf (1M MgCl2 made 8/21/07) | |||
*To correct the GTP | |||
** 5 ul MgCl2 | |||
** 50 ul resuspended GTP in water (this is in the freezer) | |||
** 45 ul of cold H2O | |||
*This gives us 100ul of sodium GTP | |||
*''Hey! Guess what? Brigette found the old GPEM and GPEM + Cushion in the e-Ice bucket near the PH Meter'' | |||
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Revision as of 14:07, 26 August 2009
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To Do
Tubulin
ProcedureNote: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start
Rhodamine Tubulin
Unlabeled Tubulin
GPEM
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