User:Linh N Le/Notebook/2009/08/26: Difference between revisions
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*Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | *Once all 4 are done, then remove the rack from the LN2 and send it to the -80C freezer | ||
**Note: if you want to polymerize one for use that day, no need to flash freeze that tube | **Note: if you want to polymerize one for use that day, no need to flash freeze that tube | ||
===Unlabeled Tubulin=== | |||
*Same as above but we made 38, 5ul aliquots in the 500ul tubes | |||
*We added 200ul GPEM+cushion (180ul GPEM + 20ul cushion) to a 1mg tube of the tubulin | |||
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Revision as of 13:38, 26 August 2009
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To Do
Tubulin
ProcedureNote: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start
Rhodamine Tubulin
Unlabeled Tubulin
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