User:Linh N Le/Notebook/2009/08/14: Difference between revisions
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*Although I'm not slated to help, I'm sure I will be around and might help Brigette make a motility assay or something to show off (at the very least just run the computer or just get in the way, we'll see) | *Although I'm not slated to help, I'm sure I will be around and might help Brigette make a motility assay or something to show off (at the very least just run the computer or just get in the way, we'll see) | ||
==Hancock Paper ReHash== | |||
*So my "questions" werent really questions so I will rehash what i said with what Andy has responded with | |||
*Q1: The paper does not dissolved the casein in the BRB completely. Why do we want it to go completely into soln? | |||
**A1: Koch got it all to go into solution back at Sandia, so we should too. Caveat: Koch's "BGB" stuff may be a milk powder (like coffee creamer) that is designed to go completely into solution whereas our purified stuff does/can not | |||
*Q2:Why do we use k-casein even though the paper says its the worst for kinesin binding? | |||
*A2:We use k because it "produces" longer MT's on the surface, making Larry's program run better. Caveat: Andy overlooked the fact that k does not bind kinesin very well (the paper measures kinesin affinity by the amount of MT's stuck to the substrate and k comes in last) and is rethinking what to do at this point | |||
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Revision as of 13:23, 14 August 2009
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Hancock Paper ReHash
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