User:Linh N Le/Notebook/2009/08/14: Difference between revisions
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*Q2:Why do we use k-casein even though the paper says its the worst for kinesin binding? | *Q2:Why do we use k-casein even though the paper says its the worst for kinesin binding? | ||
**A2:We use k because it "produces" longer MT's on the surface, making Larry's program run better. Caveat: Andy overlooked the fact that k does not bind kinesin very well (the paper measures kinesin affinity by the amount of MT's stuck to the substrate and k comes in last) and is rethinking what to do at this point | **A2:We use k because it "produces" longer MT's on the surface, making Larry's program run better. Caveat: Andy overlooked the fact that k does not bind kinesin very well (the paper measures kinesin affinity by the amount of MT's stuck to the substrate and k comes in last) and is rethinking what to do at this point | ||
**[[User:Steven J. Koch|Steve Koch]] 01:08, 16 August 2009 (EDT): This is a tough question. What we'd like is robust (works every time) and uniform (works similarly every time, repeatable). I think Andy and I both got the impression that kappa casein fit the bill here. But it's worth re-examining. Andy's idea that lipids would be even better is well-aligned with the goals of repeatability and robustness--but he'll have to figure out which lipids and how (and it still may not work as well as casein(s)) | |||
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