User:Linh N Le/Notebook/2009/08/12: Difference between revisions

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*The antifade was not working very well (or Andy forgot to add it) so most of the MT's faded quickly
*The antifade was not working very well (or Andy forgot to add it) so most of the MT's faded quickly
*Also, the lipid bi-layer did not look very uniform
*Also, the lipid bi-layer did not look very uniform
==Micelles==
*Andy wanted me to look up some stuff about Micelles
*In particular, size of casein micelles and how to make them
**Found a paper all about [http://www.rdoapp.psu.ac.th/html/sjst/journal/27-1/19casein-micelle.pdf Casein Micelles]
**They state that:
***"About 80-95% of the casein in normal milk
is in the form of colloidally dispersed particles,
known as micelles (Brunner, 1977; Fox and
McSweeney, 1998), containing on a dry basis of
94% protein and 6% colloidal calcium phosphate
(CCP), which is comprised of calcium, magnesium,
phosphate, and citrate (Fox and McSweeney, 1998).
The shape of casein micelles as observed by
electron microscopy is spherical, ranging in size
from 50-500 nm in diameter (average about 120
nm) and a molecular mass from 106-109 Da"
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Revision as of 12:29, 12 August 2009

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To Do

  • Motility assay (w/lipids)

Shaker

  • Koch was unsure about the "temperature gradient" i had reported on yesterday, and I may have made a mistake when starting as such
    • What happened is I got two statements mixed up
  • To quote the manual:
    • "Depending on various conditions within the chamber, such as flask placement and size, the heat produced by growing organisms, heat losses due to liquid evaporation from flasks, etc., the display temperature may differ from the temperatures within the flasks themselves"
    • "The temperature probe measures the temperature of the air at the probe's location, near the heat exchanger return vent."

Motility

  • Andy wants to try to mix up a new batch of kinesin and retry his lipids assay
    • Oops, he's using the old stuff
  • He also wants to redo a "standard" motility assay to make sure everything is working
  • Andy has done initial testing to show that the kinesin we have and the MT's are still in good order
  • The idea now is that the lipids are too concentrated (and the layers are too thick) so we will dilute them down
    • Andy diluted the lipids from 10mg/ml to 1mg/ml using chloroform
  • Andy spun-coated a cover glass with 1/2 lipids 1/2 naked
  • Added kinesin + brb solution to the lipids and letting it sit
  • Visualized the sample and there are MT's floating around, but none seem to really be sticking to the lipids
  • The antifade was not working very well (or Andy forgot to add it) so most of the MT's faded quickly
  • Also, the lipid bi-layer did not look very uniform

Micelles

  • Andy wanted me to look up some stuff about Micelles
  • In particular, size of casein micelles and how to make them
    • Found a paper all about Casein Micelles
    • They state that:
      • "About 80-95% of the casein in normal milk

is in the form of colloidally dispersed particles, known as micelles (Brunner, 1977; Fox and McSweeney, 1998), containing on a dry basis of 94% protein and 6% colloidal calcium phosphate (CCP), which is comprised of calcium, magnesium, phosphate, and citrate (Fox and McSweeney, 1998). The shape of casein micelles as observed by electron microscopy is spherical, ranging in size from 50-500 nm in diameter (average about 120 nm) and a molecular mass from 106-109 Da"

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