User:Linh N Le/Notebook/2009/08/12: Difference between revisions
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==Motility== | ==Motility== | ||
*Andy wants to try to mix up a new batch of kinesin and retry his lipids assay | *Andy wants to try to mix up a new batch of kinesin and retry his lipids assay | ||
**Oops, he's using the old stuff | |||
*He also wants to redo a "standard" motility assay to make sure everything is working | *He also wants to redo a "standard" motility assay to make sure everything is working | ||
*Andy has done initial testing to show that the kinesin we have and the MT's are still in good order | |||
*The idea now is that the lipids are too concentrated (and the layers are too thick) so we will dilute them down | |||
**Andy diluted the lipids from 10mg/ml to 1mg/ml using chloroform | |||
*Andy spun-coated a cover glass with 1/2 lipids 1/2 naked | |||
*Added kinesin + brb solution to the lipids and letting it sit | |||
*Visualized the sample and there are MT's floating around, but none seem to really be sticking to the lipids | |||
*The antifade was not working very well (or Andy forgot to add it) so most of the MT's faded quickly | |||
*Also, the lipid bi-layer did not look very uniform | |||
*Andy wanted to mix up some Casein in BRB80, but the BRB was not going into solution | |||
**He told me that there is a procedure, entailing the gradual mixing of casein into the BRB. I tried mixing 1/3 of the amount we wanted and it still did not go into soln | |||
==Micelles== | |||
*Andy wanted me to look up some stuff about Micelles | |||
*In particular, size of casein micelles and how to make them | |||
**Found a paper all about Casein Micelles by googling "Casein Micelles" | |||
**They state that: | |||
***"The shape of casein micelles as observed by electron microscopy is spherical, ranging in size from 50-500 nm in diameter (average about 120nm) and a molecular mass from 106-109 Da" | |||
*Its also interesting to note that they say: "size distribution of the micelles is governed by the amount if available κ-caseins" | |||
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Revision as of 14:39, 12 August 2009
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To Do
Shaker
Motility
Micelles
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