User:Linh N Le/Notebook/2009/07/30: Difference between revisions
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**The display finally got to 3C. The mix is about 5-6C (Depending if you are all the way on the bottom). In comparison, the beads only is 5-7 (depending how deep you go). The water in the bead only was 7C, so I transferred it to the water one to see if that cools it better (having water as a conduction media over beads and air) | **The display finally got to 3C. The mix is about 5-6C (Depending if you are all the way on the bottom). In comparison, the beads only is 5-7 (depending how deep you go). The water in the bead only was 7C, so I transferred it to the water one to see if that cools it better (having water as a conduction media over beads and air) | ||
*The water bucket is reading 2, the bath is reading 3, but the water tube is only reading 5-6 (of course I picked it up like a dummy and tried to measure it, so i will give it a min to cool further and remeasure) | *The water bucket is reading 2, the bath is reading 3, but the water tube is only reading 5-6 (of course I picked it up like a dummy and tried to measure it, so i will give it a min to cool further and remeasure) | ||
**Remeasured, the water is 5C | |||
*Placed the 5C water back into the beads only to test the temp (at this point, Ant has been working with this icebucket and there are alot of samples) | |||
**Its 7C, the same it was in the morning before we starting monkeying around with it. At least its consistent | |||
*Added more beads to the water sample so that the amount of beads in each was about the same. | |||
**Hey! Its still 5C after adding more beads | |||
===Results=== | |||
*The water bucket keeps the samples 2C colder | |||
**Possible reasons: | |||
**#I added water | |||
**#That particular machine is better (measuring the cold block on the water one yielded 0c and the beaded was 3c) | |||
*Added water to the "dry beads" to see if it makes a difference | |||
**The display says 2C (which it had not before) and the soup is 5C. The water tube in there is still 7C. | |||
**Update, soup at 4c water at 6c | |||
*[[User:Steven J. Koch|Steve Koch]] 00:40, 31 July 2009 (EDT): Awesome study, great work! So it looks like both of them work the same and if we use water, we can get to 5C? Since the fridge is 5C, I think that's sufficient. Thanks for checking this out! We'll have to remember that if we're every being super-delicate and want closer to 0C that we should use ice water...e.g. when resuspending tubulin. | |||
==Shaker== | ==Shaker== | ||
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*M1250-9920 Universal Platform | *M1250-9920 Universal Platform | ||
**We would need to buy the clamps and stickypads seperate | **We would need to buy the clamps and stickypads seperate | ||
**ACE-1000S 1 L Erlenmeyer Clamp with spring retainer | |||
**ACE-2000S 2 L Erlenmeyer Clamp with spring retainer | |||
**M1250-9600ø Sticky Tape (16.4’ x 1.5”) | |||
**M1250-9700** Sticky Pad, 8” x 8” (20 x 20 cm) | |||
*AG-1, 1L Erlenmeyer Flask Dedicated Platform, 15 clamps | *AG-1, 1L Erlenmeyer Flask Dedicated Platform, 15 clamps | ||
*AG-2, 2L Erlenmeyer Flask Dedicated Platform, 12 clamps | *AG-2, 2L Erlenmeyer Flask Dedicated Platform, 12 clamps | ||
*M1250-9904 Platform for Sticky Pad | *M1250-9904 Platform for Sticky Pad | ||
*No prices listed, but when we figure out what we want, a price quote can be submitted | |||
*[[User:Steven J. Koch|Steve Koch]] 00:43, 31 July 2009 (EDT): I didn't follow all of this. But maybe I can help narrow it down a bit. I have always used spring clamps (a triangle-type springy-thing, I believe. They are quite strong and screw into the platform. We shake pretty vigorously, and I can't imagine using anything less sturdy (such as sticky pad). As for versatility--we definitely will want to do different sized flasks, so that will need to be an option. But whether it needs to be "universal," I don't know. Finally, since you've got that temperature probe going, you may as well check out whether the shaker achieves the correct temperature...that would be great! We're mostly likely to use it at 37C, I think. | |||
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Revision as of 21:43, 30 July 2009
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