To Do
- Clean the Autoclave
- What does it mean to "clave"?
PBS
- Moved PBS to fridge under Andy's advice to prevent things growing in it
- updated the inventory page
Autoclave
 The cleaning stuff
- Started the Cleaning Process
- Luckily we had 1 package of Chamber Brite
- A box of 10 more packages will run ~$40
Procedure
- Remove all the trays and samples from the autoclave
- Sprinkle the contents of the Chamber Brite package in a straight line front to back
- Run a 30min cycle at 273°F (fill water, set to STE, etc)
- After the pressure goes down to 0psi, open chamber and drain the water reservoir (small adjustable spigot lower left of the opening)
- Refill the reservoir (opening upper right corner of the top of the autoclave) with DI H2O
- Run another 30min cycle w/o any Chamber Brite
- After cycle drain the reservoir again (This seems a bit redundant but that's what the manual says)
- Turn off Autoclave and allow it to cool
- Remove tray holder and wipe the inside with a damp cloth
- Wipe tray holder with damp cloth and return to chamber
- Fill the chamber with a little bit of water (2-3oz), wipe away water
- Autoclave good to go
CRLS
- Andy got the itemized budget in from CRLS and wants me to google spreadsheet it and compare to receipts
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