To Do
- More OT/DNA stuff?
- Fuss with the stupid table again?
PBS Buffer
- I made 1L of PBS buffer
- 0.01 M phosphate buffer
- 0.0027 M potassium chloride
- 0.137 M sodium chloride
- pH 7.47
- This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area
update
- So the PBS exploded in the autoclave, leaving alot of salt everywhere
- I would say about 20ml of volume was lost
- Koch worries that if it was water that evaporated instead of actual liquid splashing, then we could have a problem with concentrations
- There is salt residue everywhere so i am lead to believe that its mostly the liquid and that the concentration is ok
- I will need to clean the autoclave and we do have special autoclave cleaning powder stuff
- Tomorrow I will give it a really good cleaning, removing water and all that jazz
- We may want to look into buying more cleaning powder stuff too
Heat Plate
- Andy asked me to take a look at the Hot Plate from Fischer that died quite a while ago
- He took it apart and could not figure it out (which worries me since he's the mechanical genius in these here parts)
- Right now, what I see is that there are 2 variable resistors that go into a transformer that is powered by the AC outlet.
- One of the resistors goes to the heating element and the other goes into a motor that spins a pair of square magnets
- Brigette has taken it apart mostly and cleaned the connections
- I'm trying to look up the 2 controller knobs with not very much luck
- Perhaps I will dig around in the filing cabinet and see if the manual has anything important to say
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