User:Linh N Le/Notebook/2009/07/16: Difference between revisions

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==PBS Buffer==
==PBS Buffer==
* I made 1L of PBS buffer
* I made 1L of PBS buffer
* I used tablets supplied by [Sigma http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=P4417|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC]
*0.01 M phosphate buffer  
* One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.
*0.0027 M potassium chloride  
*0.137 M sodium chloride
*pH 7.47
**This is 1x PBS
**This is 1x PBS
*This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area
*This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area

Revision as of 14:32, 16 July 2009

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To Do

  • More OT/DNA stuff?
  • Fuss with the stupid table again?

PBS Buffer

  • I made 1L of PBS buffer
  • 0.01 M phosphate buffer
  • 0.0027 M potassium chloride
  • 0.137 M sodium chloride
  • pH 7.47
    • This is 1x PBS
  • This needs to be stored @ room temp, so I will put it with the Popping buffer near Brigette's work area