User:Linh N Le/Notebook/2009/07/13: Difference between revisions
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== | ==To Do== | ||
* | * Autoclave stuff today! | ||
** Pipette tips (1000ul) that fell out, they are sitting in the autoclave | |||
** More centrifuge tubes (the large ones) | |||
** Popin buffer | |||
* More BNC Cables | |||
* OT? | |||
==Autoclaving== | |||
*When I came in, the Autoclave was in use | |||
**Andy says that Nathan (Osinski's Lab) was using it | |||
*Round one (probably the only round since our group meeting is today) | |||
**1x 1000ul tips (the ones i got dirty Friday) | |||
**2x 100ul tips | |||
**5x large centrifuge tips | |||
***''Note: I went ahead and autoclaved ALL of the tubes in the plastic bag since we only had 1 more container of them and I wanted to make sure we had some in reserve | |||
*Round 2 | |||
** The "popin buffer" | |||
==BNC== | |||
* Whilst waiting for the Autoclave, I will make some 3ft and 4 ft BNC cables | |||
*one 4ft made friday, one made today | |||
*two 3ft made today | |||
==New Lab Network== | |||
*Used Koch's laptop to check the 4 ports that are in the new lab (2 at front door 2 at rear door) | |||
**They all provided me with internet access and I was able to access //controller | |||
*I will take down the annoying wire tomorrow since today I need to read up on the paper for the meeting | |||
==OT== | |||
*Koch, Ant and I tethered some DNA and tried unzipping | |||
*There is a problem with the translator/piezzo | |||
**They seem to be misaligned because we get alot of "sliding" | |||
***Ex. the x translation works fine, but when you use the Y translator it has a diagonal movement wrt the microscope plane | |||
* We were able to find some tethers and pull on them | |||
**We feel that the trap is too weak since the beads keep coming out of the trap | |||
**it could also be a problem with the piezzo (misaligned and/or moving too fast) | |||
*Good note: 500nm beads are easy to see and very easily trapped (sometimes we get them when we dont want them) | |||
*We need to calibrate the tweezers tomorrow | |||
*Cool note: I think i saw dna unzip, but that result was unrepeatable | |||
**We turned on the trap near a tether, saw it move, then turned off the trap. | |||
***The bead "sprung back" to its original spot and oscillated a bit (like a block on a spring) | |||
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Revision as of 11:14, 14 July 2009
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To Do
Autoclaving
BNC
New Lab Network
OT
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