User:Linh N Le/Notebook/2009/07/07: Difference between revisions

From OpenWetWare
< User:Linh N Le‎ | Notebook‎ | 2009‎ | 07
Jump to navigationJump to search
Line 35: Line 35:
**Add 99ul of BRB80T for a total of 100 ul of tubulin
**Add 99ul of BRB80T for a total of 100 ul of tubulin
***note: the  BRB80T is cold in the 4c fridge, so i warmed the tube by hand for ~5mins
***note: the  BRB80T is cold in the 4c fridge, so i warmed the tube by hand for ~5mins
**Tubes + brb80t created at 12:10pm
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}

Revision as of 11:07, 7 July 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Camera stuff
  • Kinesin activity assay?

Camera

  • Koch wants to use the camera as a gel camera.
  • He wonders how well it works wired
    • The answer to that is very well
  • The camera can dump all the photos it has taken with the push of one button
    • The destination folder on Larry's computer is in the C: drive
    • It also sub categorizes the photos by date taken
  • As for a long usb cable, Im pretty sure they make em almost as long as you would ever want
    • The camera uses usb mini-b, so we may want to get an adapter (the thought being get a really long usb A cord and get an A to mini b adaptor, that way the long cord can be used for other things as well)
  • Amazon has usb A extension cables (15-20ft) for under $10

Kinesin Activity Assay

  • Brigette wanted to try to do the phosphorous assay kit that we got from cytoskeleton
  • From what I know about this, we mix up tubes and ATP and let it sit at different times and measure the phosphorus levels
  • We then mix our kinesin and the tubes and ATP, let sit at diff times, and re measure the phosphorus
  • Right now, we are going to polymerize some non-labeled tubes
  • Brigette is writing up some procedures which I hope to copy from her and paste in my notebook

Procedure

  • Taking note of what we did as we do it:
  • 1 aliquot of the unlabeled tubes
    • They are in the -80c in the large falcon tubes
  • Polymerize them for 20min @ 37C
    • Add 99ul of BRB80T for a total of 100 ul of tubulin
      • note: the BRB80T is cold in the 4c fridge, so i warmed the tube by hand for ~5mins
    • Tubes + brb80t created at 12:10pm
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Linh_N_Le/Notebook/2009/07/07&oldid=322128"