User:Linh N Le/Notebook/2009/07/07: Difference between revisions
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==Camera== | ==Camera== | ||
{{SJK Comment|l=23:04, 7 July 2009 (EDT)|c=That's good to know that it works really well wired. One really important function is the remote camera control: Taking a picture while controlling and viewing from the computer. Does this work well over the wired?<br>[[User:Linh N Le|Linh N Le]] 01:08, 8 July 2009 (EDT)There is a button on the software that is supposed to control the camera remotely, but while it is wired, the button is un-clickable.}} | |||
*Koch wants to use the camera as a gel camera. | *Koch wants to use the camera as a gel camera. | ||
*He wonders how well it works wired | *He wonders how well it works wired | ||
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*Amazon has usb A extension cables (15-20ft) for under $10 | *Amazon has usb A extension cables (15-20ft) for under $10 | ||
**[http://www.amazon.com/Extension-Cable-Type-Male-Female/dp/B001847JD8/ref=sr_1_8?ie=UTF8&s=electronics&qid=1246986611&sr=8-8 15ft male A to female A extension] | **[http://www.amazon.com/Extension-Cable-Type-Male-Female/dp/B001847JD8/ref=sr_1_8?ie=UTF8&s=electronics&qid=1246986611&sr=8-8 15ft male A to female A extension] | ||
*So Koch ordered two Canon Cameras and wants me to try to get the second one setup | |||
===Round 2=== | |||
*I am uninstalling the software from last time b/c I had trouble installing it the first go | |||
**Actually, having a hard time uninstalling the software...stupid Canon | |||
*Ok, all uninstalled...lets see if it will let me reinstall all the software | |||
**Had a problem yesterday of installing and then the program crashed. It must have left some residue behind since it says that everything is all nice and installed, yet it was not there | |||
***The "Photostitch" program is buggy. It tells me that it has already been installed, then crashes the installer when it fails. I can get around this by intalling manually and then telling it ''not'' to mess with photostitch | |||
*It seems that I have the "essential" software installed | |||
*Looks like it does not come with a memory card, hope thats ok | |||
**The new battery is uncharged, using one from old cam | |||
*Setting up wireless | |||
**SSID Kochlab | |||
**security WPA-PSK-TKIP | |||
**key [[User:Andy Maloney|Andy Maloney]] 00:17, 8 July 2009 (EDT): <font color = red>Really? You put the key on the public wiki?</font> I know we are an open lab but don't you think that's a bit much? | |||
***[[User:Linh N Le|Linh N Le]] 01:07, 8 July 2009 (EDT)Oh shoot! I'm sorry, i totally spaced that I did that. I just wanted to document what I wrote down so that if the camera didn't connect it could have been traced to a bad password. I gotta keep reminding myself that this is a ''public'' wiki | |||
*Got that setup but when trying to connect this battery also died | |||
**May need to wait for a charge tomorrow | |||
==Kinesin Activity Assay== | ==Kinesin Activity Assay== | ||
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*Right now, we are going to polymerize some non-labeled tubes | *Right now, we are going to polymerize some non-labeled tubes | ||
*Brigette is writing up some procedures which I hope to copy from her and paste in my notebook | *Brigette is writing up some procedures which I hope to copy from her and paste in my notebook | ||
==Procedure== | ===Procedure=== | ||
*Taking note of what we did as we do it: | *Taking note of what we did as we do it: | ||
*1 aliquot of the unlabeled tubes | *1 aliquot of the unlabeled tubes | ||
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*** 30ul MT + 30ul Kin + 1.2ul ATP (2uM ATP) | *** 30ul MT + 30ul Kin + 1.2ul ATP (2uM ATP) | ||
*** 30ul MT + 30ul BRB80T + 1.2ul ATP (2uM ATP) | *** 30ul MT + 30ul BRB80T + 1.2ul ATP (2uM ATP) | ||
***''Note: When i say MT I mean MT+AF | ***''Note: When i say MT I mean MT+AF (motillity soln w/o casein) | ||
***''Second Note: some drops got stuck to the side walls of the tubes, so Brigette washed them down with a couple ul of BRB80 | |||
*We let the samples sit for ~10mins to allow the reactions to complete (since adding the dye and quencher stop the reactions all together) | |||
*Turns out it is over diluted and Brigette didn't get any useful readings from it | |||
====Trial 2==== | |||
*We are redoing it, but w/o any naked brb80 | |||
*Samples: | |||
*** Motility Assay alone | |||
*** Mot +1uM ATP | |||
*** Mot +2 ATP | |||
*** Mot+ 3 ATP | |||
*** Mot | |||
*** Mot+Kin | |||
*** Mot+kin+ATP | |||
*** Mot+kin+2ATP | |||
*** Mot+Kin+3Atp | |||
*Allowing it to sit and react for ~ 30mins | |||
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Revision as of 22:08, 7 July 2009
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To Do
CameraSJK 23:04, 7 July 2009 (EDT)
Round 2
Kinesin Activity Assay
Procedure
Trial 2
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