User:Linh N Le/Notebook/2009/06/23

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To Do

  • Koch/Atlas group meeting at 3
  • Try out new buffer we made yesterday?
  • Try Kinesin?
  • Clean up lab

BRB80

  • Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
    • People to look for, Block and Vale

Recipes

  • Hancock (Used by us as well)
    • 80 mM PIPES pH 6.85 (we use ph6.9)
    • 1 mM MgCl2
    • 1 mM EGTA
  • T.Salmon Labs
    • PM Buffer
      • 100 mM PIPES, pH 6.9
      • 2 mM EGTA
      • 1 mM Mg2SO4
  • Goldman Lab (Northwestern University)
    • 100 mM Pipes,pH 6.9
    • 1 mM MgCl2
    • 1 mM EGTA
  • Cold Spring Harbor Labs
    • PEM Buffer
      • 0.1 M PIPES (pH 6.95)
      • 2 mM EGTA
      • 1 mM MgSO4
  • Cytoskeleton
    • PEM (General Tubulin Buffer)
      • 80 mM Na-PIPES pH 6.9
      • 1 mM MgCl2
      • 1 mM EGTA
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