User:Linh N Le/Notebook/2009/06/23: Difference between revisions

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***20 μM taxol  
***20 μM taxol  
***10% v/v DMSO)
***10% v/v DMSO)
**PEM80  
**Assay buffer based on PEM80  
***80 mM PIPES at pH 6.9
***80 mM PIPES at pH 6.9
***1 mM EGTA  
***1 mM EGTA  

Revision as of 16:17, 23 June 2009

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To Do

  • Koch/Atlas group meeting at 3
  • Try out new buffer we made yesterday?
  • Try Kinesin?
  • Clean up lab

BRB80

  • Since there are so many ways to make this buffer, Koch wanted me to try to find different famous people's recipes for the buffer
    • People to look for, Block and Vale
  • What we use for Cytoskeleton is called PEM and while PEM and BRB80 can be the same, they do not have to be
    • I did searches on both PEM and BRB80 and the following recipes are also reported by what they are called
  • Special Note: All PDF's linked here are from the personal website of the authors and are free access

Recipes

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