User:Linh N Le/Notebook/2009/06/23: Difference between revisions
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**People to look for, Block and Vale | **People to look for, Block and Vale | ||
===Recipes=== | ===Recipes=== | ||
*Hancock (Used by us as well) | *[http://www.proweb.org/kinesin/Methods/motility.html Hancock (Used by us as well)] | ||
**80 mM PIPES pH 6.85 (we use ph6.9) | **80 mM PIPES pH 6.85 (we use ph6.9) | ||
**1 mM MgCl2 | **1 mM MgCl2 | ||
**1 mM EGTA | **1 mM EGTA | ||
*T.Salmon Labs | *[http://www.proweb.org/kinesin/Methods/MT_assembly.html T.Salmon Labs] | ||
**PM Buffer | **PM Buffer | ||
***100 mM PIPES, pH 6.9 | ***100 mM PIPES, pH 6.9 | ||
***2 mM EGTA | ***2 mM EGTA | ||
***1 mM Mg2SO4 | ***1 mM Mg2SO4 | ||
*[www.goldmanlab.northwestern.edu/protocols. | *[www.goldmanlab.northwestern.edu/protocols.htm Goldman Lab (Northwestern University)] | ||
**100 mM Pipes,pH 6.9 | **100 mM Pipes,pH 6.9 | ||
**1 mM MgCl2 | **1 mM MgCl2 | ||
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***2mM EGTA | ***2mM EGTA | ||
**Notes: BRB80 is good for microtubules and 'cytoskeleton buffer' is good for both actin filaments and microtubules | **Notes: BRB80 is good for microtubules and 'cytoskeleton buffer' is good for both actin filaments and microtubules | ||
*[http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibodies-application/protocols/immunofluorescence.html Sigma Aldrich] | |||
**PEM Buffer: | |||
***0.1M PIPES (Product No. P8203) | |||
***5 mM EGTA (Product No. E4378) | |||
***2mM MgCl2 · 6H2O (Product No. M0250) | |||
***Bring to pH 6.8, using NaOH solution | |||
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Revision as of 13:59, 23 June 2009
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To Do
BRB80
Recipes
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