To Do
- Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed
- Make BRB80
Tubulin
- Stumbled upon a site that details how they get tubulin from cow's brains
BRB80
- Since Andy and Koch are the only people in the lab that know how to make a buffer (I actually dont know if Ant can), we waited til Andy came back to try to make BRB80
- Not to mention the fact that we are also running out of the stuff we got from Cytoskeleton (new shipment will be in this week)
- Andy is looking over our new fancy Ph meter
- BRB stands for Britton-Robinson Buffer
- It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it
- It consists of:
- .04M H3BO3 (Boric Acid)
- .04M H3PO4 (Phosphoric Acid)
- .04M CH3COOH (Acetic Acid)
- The stuff we use is also known as PEM
- 0.1 M PIPES (pH 6.95)
- Piperazinediethanesulfonic acid C8H18N2O6S2
- 2 mM EGTA
- ethylene glycol tetraacetic acid C14H24N2O10
- 1 mM MgSO4
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