User:Linh N Le/Notebook/2009/06/22: Difference between revisions
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* Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed | * Andy is back! He wants to try the kinesin again, maybe his expertise will allow us to succeed | ||
* Make BRB80 | * Make BRB80 | ||
12-5 | |||
==Tubulin== | ==Tubulin== | ||
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*Andy is looking over our new fancy [[Koch Lab:Inventory/pH meter|pH meter]] | *Andy is looking over our new fancy [[Koch Lab:Inventory/pH meter|pH meter]] | ||
**He is starting the calibration process | **He is starting the calibration process | ||
*BRB stands for [http://en.wikipedia.org/wiki/Britton-Robinson_buffer Britton-Robinson Buffer]{{SJK Comment|l=01:52, 23 June 2009 (EDT)|c=I can't tell if you're kidding! Check out this friendfeed thread: [http://friendfeed.com/the-life-scientists/59d2ea57/does-anyone-here-know-what-initials-brb80-stand what is BRB80?]}} | *BRB stands for [http://en.wikipedia.org/wiki/Britton-Robinson_buffer Britton-Robinson Buffer]{{SJK Comment|l=01:52, 23 June 2009 (EDT)|c=I can't tell if you're kidding! Check out this friendfeed thread: [http://friendfeed.com/the-life-scientists/59d2ea57/does-anyone-here-know-what-initials-brb80-stand what is BRB80?]<br> | ||
[[User:Linh N Le|Linh N Le]] 19:31, 24 June 2009 (EDT)I googled "BRB Buffer" and thats what I found. I now know thats now what we want}} | |||
**It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it | **It is a "universal" buffer whose ph changes linearly with the amount of alkali that is added to it | ||
**It consists of: | **It consists of: | ||
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*We titrated it with NaOH to raise the Ph to 6.89 | *We titrated it with NaOH to raise the Ph to 6.89 | ||
**Used about 26ml of NaOH to get there | **Used about 26ml of NaOH to get there | ||
*Added an entry to the Kochlab inventory page, under the VWR fridge category | *Added an entry to the Kochlab inventory page, under the VWR fridge category ([[User:Steven J. Koch|Steve Koch]] 02:07, 23 June 2009 (EDT): Thank you! Excellent notes, as always. I really appreciate it.) | ||
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Revision as of 16:31, 24 June 2009
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To Do
12-5 Tubulin
BRB80
Ph Titrations
Making the Buffer
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