To Do
- Autoclave more tips
- Work on Kinesin if Brigette comes in
- Organize the wiki
Autoclave
- Round 1
- 1 bottle of small centrifuge tubes
- 1 bottle of large centrifuge tubes
- 2 boxes 1000μL tips
- 1 box 100ul tips
- 1 box 10ul tips
- Round 2
- We have no-sterilized tips for the 2.5ul pipetteman
- 6 boxes of 2.5ul (labeled 10ul) tips
- They are in the new eppindorf boxes with a black background
Hiring
- I finally got hired!
- I just need to head down to HR on Lomas and University to fill out paperwork
- I'm doing a timesheet right now
- I believe Koch and I came to an agreement that the month of May was a "work for free" period with a pay increase the rest of the summer to make up for it.
- As such, i will only "report" time from Jun1-now
Kinesin
- I polymerized a batch of the RMTs while Brigette made some kinesin
- She aliquoted ~6 tubes of kinesin (1ul ea), flash froze and stored in the -80c
- She is making the motillity assay we made last time, with the same casein (the BGB koch made).
- The first go with the kinesin was a bust
- It was different this time, as there were alot more tubes stuck to the glass than last time
- We decided to try a few things at the same time differently
- first, i made a new BGB using the old powder and even older brb80 that koch made 5 yrs ago
- We used that to coat the glass
- Second, Brigette was afraid that there wasnt enough ATP so she tripled the concentration
- We did 2 samples, one with same kinesin + super atp
- old kinesin + super atp + more BRBCT
- The thinking here was that Brigette made a too high on kinesin concentration and so we diluted it some more
- Suffice it to say, there was not very much change in what the samples looked at from earlier.
- I did take a video of tubes bleaching and exploding!
What could have went wrong
- Besides the obvious "we messed it up"
- The BGB could be bad
- doubtful because Koch has made assays with BGB before
- Brigette mentions that Koch's recipe for kinesin was off the top of his head, so maybe koch was wrong? (Koch wrong?! Blasphemous!!!)
- The kinesin could be terribly slow and not see-a-ble
- on that note: Brigette used the "leftovers" of the vial, so that could also be an issue
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