User:Linh N Le/Notebook/2009/06/19: Difference between revisions
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*The BGB could be bad | *The BGB could be bad | ||
**doubtful because Koch has made assays with BGB before | **doubtful because Koch has made assays with BGB before | ||
**[[User:Steven J. Koch|Steve Koch]] 22:05, 19 June 2009 (EDT):I'll have to dig around, but I'm not sure I've done motility assays with BGB (it works great for DNA tethering, but not sure about motility--not out of the question that it is bad for motility) | |||
*Brigette mentions that Koch's recipe for kinesin was off the top of his head, so maybe koch was wrong? (Koch wrong?! Blasphemous!!!) | *Brigette mentions that Koch's recipe for kinesin was off the top of his head, so maybe koch was wrong? (Koch wrong?! Blasphemous!!!) | ||
**[[User:Steven J. Koch|Steve Koch]] 22:02, 19 June 2009 (EDT): Ha! Doubting Koch is good science! In this case, I'm not sure what recipe she's talking about. The printed recipe is mostly written by people at Sandia, and based on some standard protocols (available on [http://www.proweb.org/kinesin/Methods/motility.html "the kinesin home page"]. There's no doubt we're cutting some corners here as we start up, and we'll keep working to take on more of those steps (such as making BRB80, which we should do next week). | **[[User:Steven J. Koch|Steve Koch]] 22:02, 19 June 2009 (EDT): Ha! Doubting Koch is good science! In this case, I'm not sure what recipe she's talking about. The printed recipe is mostly written by people at Sandia, and based on some standard protocols (available on [http://www.proweb.org/kinesin/Methods/motility.html "the kinesin home page"]. There's no doubt we're cutting some corners here as we start up, and we'll keep working to take on more of those steps (such as making BRB80, which we should do next week). | ||
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==Incubator== | ==Incubator== | ||
*The temp was 27.6°C when i left at 6pm | *The temp was 27.6°C when i left at 6pm | ||
**[[User:Steven J. Koch|Steve Koch]] 22:04, 19 June 2009 (EDT): Thanks for noting this! | |||
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Revision as of 19:05, 19 June 2009
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To Do
Autoclave
Hiring
Kinesin
What could have went wrong
Incubator
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