User:Linh N Le/Notebook/2009/06/17/How to use the Autoclave: Difference between revisions
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(New page: ==Initial Check== *On the top of the autoclave is a round opening for DI water *The cap has a dipstick in it where you can check water levels **Note: do not over fill the autoclave. There ...) |
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*Wait for the pressure to reach 0 psi | *Wait for the pressure to reach 0 psi | ||
*Open the door slightly | *Open the door slightly | ||
*Set the bottom-most dial to "EXH + DRY" | |||
*Set the timer knob to 20mins | *Set the timer knob to 20mins | ||
**The DRY light should be on now | |||
*When the timer expires, change the bottom most knob to the "0" position | *When the timer expires, change the bottom most knob to the "0" position | ||
*Open door and remove samples | *Open door and remove samples | ||
Revision as of 16:16, 17 June 2009
Initial Check
- On the top of the autoclave is a round opening for DI water
- The cap has a dipstick in it where you can check water levels
- Note: do not over fill the autoclave. There is a metal spigot inside the filling area that the water level must not rise above
Preparing samples
- Tubes should be placed in the round plastic screw cap containers
- The cap should be placed upon the top, but not screwed in
- Pipette tip boxes should be opened and their lids on, but not airtight
- All samples must have the special masking tape on them
- This tape is the manilla with white stripes on it
- Make a small tab at one end so that the tape can be easily removed for reuse of the container.
- Place the samples into the tray of the autoclave, evenly spaced apart
Setup
- After the autoclave has been filled with water and loaded, turn on the green switch to "START"
- Turn the bottom-most dial clockwise to the "FILL WATER" position
- Watch inside the autoclave bottom as water starts to fill it
- When the water reaches the door (There is a small notch before the door) set the bottom dial to "STE"
- Close door and tighten (not too tight because you may damage the seal)
- With the door closed, set the temperature gauge to 273F (121C)
- Set the timer to 30mins (45mins if this is a cold start)
- Note: the pressure meter must be between 30-32 psi for the temperature to actually be 273F. If it is not, adjust the temperature gauge accordingly
Drying
- After the timer is done, a small "ding" will be heard
- Wait for the pressure to reach 0 psi
- Open the door slightly
- Set the bottom-most dial to "EXH + DRY"
- Set the timer knob to 20mins
- The DRY light should be on now
- When the timer expires, change the bottom most knob to the "0" position
- Open door and remove samples
No drying
- After the timer dings set the bottom most knob to "EXH + DRY"
- When the pressure gauge reaches 0 psi, turn the bottom most knob to the "0" position
- Open door and remove samples
