LN2 Container
- Working with LN2 yesterday to flash freeze the tubulin made us realize that a container to transport it into the lab would be much more useful
- Very apparent when trying to haul it in with the flat, shallow, red ice bucket and having it almost spill everywhere
- For $3-500, it did not seem like a worthy investment
- Instead, today, we will take the black ice buckets and fill them with LN2, then transfer it in the lab as needed
- useful the the spigot is only ~2m away from the back door
- Possible to get a feed inside the lab? (just a thought)
Videos from yesterday
- I am moving them to the webpub as koch advised me to.
- Moving to \\controller\webpub\files\movies\MT movies
- Should be accessible via kochlab.org
Video Card
- The subject of videos reminds me that I should go and make sure Tamra gets my order in
- I guess i'm really good at catching her when she's not around
- will send an email instead
KochLab Inventory
- Koch wants me to go over the inventory page and see what else needs to be inventoried
- something we may want to add as inventory are:
- Wetlab shelves
- The fridge (VWR next to -80c freezer)
- Fume hood
- I guess that means I will need to make new templates to go with the new inventory areas
Wetlab shelves
- I want to me a naming convention for these shelves
- Looking at the shelves with your back to the main sink towards the fume hood
- Upper left 1, below that 2, upper right 3, below that 4
- please also mention if it is in front or back
- i.e ph7 buffer Wetlab shelf 2 front
New Templates
- I made 3 new templates for the inventory page
- Mark a page with the following templates to indicate where it is
- example marking {{KL 80|b=shelf 2}}
- Reorders {{Reorder}}{{KL Inv}}
- -20c {{KL 20}}
- -80c {{KL 80}}. Also include |b=black or |b=shelf 2 for location
- VWR fridge {{KL VWR}}. Also include |s=left or |s=right to indicate the side of the fridge it is in
- Wet Lab {{KL WL}}. Also include |s=1 or |shelf=2 for shelf spot (see convention above)
- Fume Hood {{KL FH}}
Kinesin + tubes
- we polymerized another aliquot of the RMTs
- Koch and brigette made a new stock of BRB80T
- I tested the tubes with an old AF we used yesterday and refroze
- from what we can tell, it works ok, but not as good
- fades faster + there are broken tubes floating around
- thawing a new AF to test this theory
- koch has also made the new computer the camera computer, so we are playing with that now
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