User:Linh N Le/Notebook/2009/06/16: Difference between revisions
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**useful the the spigot is only ~2m away from the back door | **useful the the spigot is only ~2m away from the back door | ||
***Possible to get a feed inside the lab? (just a thought) | ***Possible to get a feed inside the lab? (just a thought) | ||
****[[User:Steven J. Koch|Steve Koch]] 23:54, 16 June 2009 (EDT): Yes, that may be possible. Definitely $300 is worth it if safety is an issue. As I see it now, a couple trips with small bucket seems reasonable and safe. But let me know if not! | |||
==Videos from yesterday== | ==Videos from yesterday== | ||
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*thawing a new AF to test this theory | *thawing a new AF to test this theory | ||
*koch has also made the new computer the camera computer, so we are playing with that now | *koch has also made the new computer the camera computer, so we are playing with that now | ||
**Works as intended | |||
===kinesin=== | |||
*Koch and brigette worked out the formula for the motility assay | |||
*I dont have the exact details, they are in Koch's notebook (i will link later) ([[User:Steven J. Koch/Notebook/Kochlab/2009/06/16/Motility assays]]) | |||
*They made the casein solution (From the powdered milk) and the motility soln | |||
*The kinesin that Koch used was some old stuff from ~5years ago | |||
*We made a dual chamber slide | |||
*One had casein + motility soln (tubes, af, atp, etc) + kinesin | |||
*The other only has motility soln (i cannot remember if both had casein in it) | |||
**The control (tubes only) showed us tubes that were stuck mostly to the glass | |||
***on that note, i dont think the control had casein | |||
**The kinesin side had alot more loose tubes and alot more tubes that were only stuck at one end and flopped around. | |||
**The ones that were stuck to the glass we watched. It did not seem that they were moving at all | |||
*Trying again, we made another dual chamber (note: makes sure the channels run along the length of the slide, it makes things much easier, especially when looking with the microscope. | |||
**One chamber had the original kinesin mixture as a control | |||
**The second chamber had ~10x more kinesin | |||
***I personally only got to look at the 10x sample, but brigette says the 1x looks exactly the same | |||
***The 10x seemed to have much more "stuck" tubes (That is not moving around and completely stuck to the glass) | |||
***Other than that, i saw no movement | |||
*Some thoughts | |||
**Is the casein coating all the glass? I know that tubes dont like casein, but maybe they are sticking on areas that dont have it coated on there | |||
**Will kinesin bind tubes, even if the kinesin wont walk? My thoughts are no, but the idea came up with the 10x concentration. Perhaps the kinesin are in the casein and are acting like velcro, just sticking MT's down | |||
**No huge surprise that 5yo kinesin stored at -20c (instead of -70c) doesnt work too well | |||
*Movies were made, I will upload these to the webpub with the ones we made yesterday! | |||
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Revision as of 20:55, 16 June 2009
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Kinesin + tubes
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