User:Linh N Le/Notebook/2009/06/15: Difference between revisions
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*[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_(Rhodamine)_and_regular_tubulin Koch's notes] | *[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_(Rhodamine)_and_regular_tubulin Koch's notes] | ||
*The AF works great. The tubes stay in the field of view for over 5mins. | *The AF works great. The tubes stay in the field of view for over 5mins. | ||
**We took video of the tubes looking for any breaks (or other destruction of MTs), but saw nothing. Videos on larry's computer in | **We took video of the tubes looking for any breaks (or other destruction of MTs), but saw nothing. Videos on larry's computer in C:\Movies from Live Feed\avi\herskowitz.larry\09Jun15 under various names | ||
**Moved to Z drive. pub\,dropzone\MT videos\Rhodamine jun15 | |||
**[[User:Steven J. Koch|Steve Koch]] 23:48, 15 June 2009 (EDT): You can put them in webpub and then they will be accessible via regular public internet. | |||
*Going to try vitamin C next and then vitamin C + AF | *Going to try vitamin C next and then vitamin C + AF | ||
**Honestly, I dont see the point of vitamin C now. The taxol we add is supposed to stabalize the tubes. Perhaps there is a long term effect (10mins+) that vitamin C helps with | **Honestly, I dont see the point of vitamin C now. The taxol we add is supposed to stabalize the tubes. Perhaps there is a long term effect (10mins+) that vitamin C helps with | ||
*The Ascetic Acid seems to work just fine. Brigette mixed it with the stock MTs and there are alot of tubes. It gets a bit overwhelming, but they are just as apparent as they were w/o the AA. | |||
*Ascetic Acid + AF is still really bad. The AF doesn't prevent bleaching as strongly anymore and there are no tubes to be found | |||
*Cool note: around 1min mark of the first video, you can see a MT loose, then attaching itself to the glass (center screen) | |||
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Revision as of 20:48, 15 June 2009
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Video Card
Linh N Le 14:20, 15 June 2009 (EDT) Order sent in with Tamara. Actually left it on her door, i think shes out to lunch MTs
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