Questions for Andy
- Andy asked Brigette and I to come up with questions pertaining the experiments we ran last night (AA + MT + AF)
- The bulk of the questions, for me at least, will deal with some chemistry and Ph related things
The Questions
- What Ph do MT's thrive in? (my guess would be near 7)
- What is the Ph of BRB80 (and BRB80T)? (I overheard it was made of salts, so possibly near 7 also)
- For that matter, what is BRB80 comprised of?
- What is the Ph of the AF cocktail? ( I should look up Glucose oxidase (GOD), Catalase (CAT), and beta-mercaptoethanol (BME))
- How does Ascetic Acid and AF interact? (it seemed to do terrible things to the tubulin)
- For that matter, how does AF interact with the MT's?
The Chemicals
- Glucose Oxidase is an enzyme that binds to beta-D-glucopyranose (a form of the sugar glucose)
- It is dimeric
- It uses flavin adenine dinucleotide (FAD), which comes from Redox reactions, to become a catalyst.
- It also reduces natural 02 into h202 (hydrogen peroxide)
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