User:Linh N Le/Notebook/2009/06/12: Difference between revisions
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== | ==Questions for Andy== | ||
* | * Andy asked Brigette and I to come up with questions pertaining the experiments we ran last night (AA + MT + AF) | ||
* The bulk of the questions, for me at least, will deal with some chemistry and pH related things | |||
===The Questions=== | |||
# What pH do MT's thrive in? (my guess would be near 7) | |||
# What is the pH of BRB80 (and BRB80T)? (I overheard it was made of salts, so possibly near 7 also) | |||
# For that matter, what is BRB80 comprised of? | |||
# What is the pH of the AF cocktail? ( I should look up Glucose oxidase (GOD), Catalase (CAT), and beta-mercaptoethanol (BME)) | |||
# How does Ascetic Acid and AF interact? (it seemed to do terrible things to the tubulin) | |||
# For that matter, how does AF interact with the MT's? | |||
==The Chemicals== | |||
*Glucose Oxidase is an enzyme that binds to beta-D-glucopyranose (a form of the sugar glucose) | |||
*It is dimeric | |||
*It uses flavin adenine dinucleotide (FAD), which comes from Redox reactions, to become a catalyst. | |||
*It also reduces natural 02 into h202 (hydrogen peroxide) | |||
==Computer== | |||
*Koch and I got the drivers installed and the computer put on the server | |||
*Its name is Kochlab-daq2 | |||
*It has 2 ports in the back (as far as I can tell) one for a video card and one for a DAQ card | |||
*LABView 71 and visual safe source also installed | |||
===Video Cards=== | |||
*I went to the Dell site and tried to "build my own" optiplex 960 | |||
*I figure if Dell sells the card, they work with the computer. I will also try to price them out on sites through Google shopping | |||
*Here are the video cards they offer: | |||
**256MB ATI RADEON HD 3450 (Dual DVI/ VGA /1 TV-out), full height [add $74] | |||
***Amazon:~$40 | |||
**256MB ATI RADEON HD 3470 (Dual DP), full height [add $84] | |||
***$70-120 | |||
**512MB ATI RADEON HD4670,Dual DVI/ plus single DVI VGA adapter [add $139] | |||
***~$60-70 | |||
**256MB nVidia GeForce 9300 GE (Dual DVI/ VGA /1 TV-out), full height [add $74] | |||
***Amazon: 50-60 | |||
**512MB NVIDIA Quadro NVS 420 Quad DVI Adapter, Full Height [add $499] | |||
***Amazon: ~480 | |||
**512MB NVIDIA Quadro NVS 420 Quad DisplayPort Adapter, Full Height [add $499] | |||
***Amazon: same as above | |||
*So, any descent card will def be under 100, and we can just ship it in and take 20mins to install it to the computer itself | |||
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Revision as of 20:17, 12 June 2009
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Questions for Andy
The Questions
The Chemicals
Computer
Video Cards
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