User:Linh N Le/Notebook/2009/06/11: Difference between revisions
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**~45mins after creation, I took a look a new sample of the AF+MT(3ulaa)+dex+brb80t+2ulaa mix and saw some floaties. | **~45mins after creation, I took a look a new sample of the AF+MT(3ulaa)+dex+brb80t+2ulaa mix and saw some floaties. | ||
**The AF didnt seem to work very well. The background went black too fast! | **The AF didnt seem to work very well. The background went black too fast! | ||
*Getting frustrated at the fact we could not see anything, I went ahead and sampled out the pure tubes +1.5ul aa | |||
**We saw plenty of tubes, and even though they do fade quite rapidly, you can still see them faintly in the background | |||
*Conclusion of the day: AF does not screw up MT. AA does not screw up MT. Mixing the 2 messes everything up! | |||
*In a last ditch effort, Brigette added 10 more ul of MT(with 3ul of AA) to the AF mixture | |||
**Hopefully more MT's allows us to see some | |||
*On a side note: we really need another microscope as Andy came back into the lab and wants to work on the tweezers, but we are still looking at samples! | |||
*MT's, no antifade, with aa, can see tubes. MTs with AF, with aa, still fades badly, but not tubes. "bad" AF that doesnt stop fading, +MTs, can see tubes, but they fade fast (as we saw last week). MTs + aa +AF (whether or not its good b/c it was in the freezer, or even if I messed it up by not thawing it right) no tubes. aa+af=bad for tubes. | |||
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